Device

Part:BBa_K2008001:Design

Designed by: Rachelle Varga   Group: iGEM16_UofC_Calgary   (2016-10-08)


BBI with N-terminal KSCI solubility tag fused to GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 742


Design Notes

We used a strong RBS and constitutive promoter to produce high levels of expression of the BBI-KSCI tagged protein fused to GFP for visualization.


Source

Members of the lentil family produce the full BBI protein, and the genetic sequence for the peptide was sourced originally from genomic DNA.

References